Supplementary Figure 4: Registration error
From: An interactive framework for whole-brain maps at cellular resolution

(a) Regions of interests for measuring registration error before and after manual landmark correction. Regions were selected based on their difference in proximity to the center of the tissue section. Scale bar 1 mm. (b) Root Mean Square Error (RMSE) in micrometers as a function of brain nuclei in four different brains and 64 landmarks along the contour of each nuclei. (c) Mean RMSE as a function of average distance from center of tissue section.