Supplementary Figure 5: Comparison of quantifications at different sectioning planes
From: An interactive framework for whole-brain maps at cellular resolution
(a-f) Representative sections (n = 6) from a total of 53 imaged and quantified sections representing a single mouse brain stained for FOXP2. Left: Original fluorescent image with atlas fit superimposed (purple). Right: segmented FOXP2+ nuclei transformed into stereotactic reference atlas where individual nuclei are color coded according to brain region. (g-l) Representative section (n = 6) from a total of 29 sagittal mouse brain sections also stained for FOXP2. Top: Original fluorescent image with atlas fit superimposed (purple). Bottom: segmented FOXP2+ nuclei transformed into stereotactic reference atlas where individual nuclei are color coded according to brain region. (m) 3D reconstruction of 53 individual FOXP2stained coronal sections. (n) 3D reconstruction of FOXP2 segmented nuclei obtained in the sagittal plane from a single hemisphere and then mirrored to obtain a whole-brain representation at almost half the imaging, sectioning and mounting time. (o) A more complete 3D reconstruction can be achieved by combining (m) and (n). Scale bars: 1 mm.
