Supplementary Figure 3: Comparison of the expression pattern of ZIKV using two types of antibodies and proliferation assessment.
From: Stress-induced unfolded protein response contributes to Zika virus–associated microcephaly
a-b,Immunolabelings of brain slices from E14.5 embryos infected by ZIKV after intracerebroventricular injection at E12.5 and followed by in utero electroporation of GFP at E13.5. Immunolabelings show Tbr2 (blue), Sox2 (light grey), GFP (green), and ZIKV (red, using two types of antibodies, as indicated in a and b (n = 3 independent experiments from 3 different embryonic brains). c-d, Assessment of proliferation rate (Ki67+, white) of GFP+ apical progenitors (green) in Mock condition or after ZIKV infection (red). Nuclei are counterstained with Dapi (blue) (n = 3 to 6 independent experiments) c-d. Data is presented as boxplot of median ± first to third quartiles while whiskers extend to maxima and minima with each symbol representing independent embryonic brains (n = 3 to 6, P < 0.116, U = 3), as indicated on boxplots and analyzed by two-sided Mann-Whitney test c. Scale bar represent 200 µm a, b, 100 µm (boxed regions in a and b), or 50 µm d. ns means not significant.
