Supplementary Figure 5: The Marble mouse does not show obvious defects in the dentate gyrus nor deficits in locomotion or anxiety | Nature Neuroscience

Supplementary Figure 5: The Marble mouse does not show obvious defects in the dentate gyrus nor deficits in locomotion or anxiety

From: Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans

Supplementary Figure 5

a-x Representative images of histological stainings on the dentate gyrus of adult +/+, +Mbe, and Mbe/Mbe animals with sera against Prox1 (which labels mature granule cells), GFAP (which labels radial glial-like progenitors), Dcx (which labels newly born neurons), and Tbr2 (which labels intermediate progenitors). These experiments revealed that the granule cell layer and the subgranular zone is similarly organized in all genotypes (n = 3 animals per genotype). y Total distance travelled in the open field reveals no locomotor phenotype in Mbe/Mbe animals (n = 17 animals per genotype; one-way ANOVA with a Tukey’s multiple comparisons test, Bonferroni corrected; +/+ vs Mbe/Mbe; P>0.9999). z Number of entries into the center of the open field. There is no significant difference between genotypes indicating anxiety phenotypes are normal in Mbe/Mbe animals (n = 17 animals per genotype; one-way ANOVA with a Tukey’s multiple comparisons test, Bonferroni corrected; +/+ vs Mbe/Mbe; P = 0.7546). The scale bars show 500 μm in a and 100 μm b. Error bars show mean +/- standard error of the mean.

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