Supplementary Figure 6: Anatomical characterization of the Marble cortex and cerebellum | Nature Neuroscience

Supplementary Figure 6: Anatomical characterization of the Marble cortex and cerebellum

From: Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans

Supplementary Figure 6

a-g Representative images of birth date labeling studies following the injection of BrdU in pregnant dams at E12.5 a-c and E14.5 e-g followed by histological analysis at P0. Cortical sections were divided into ten equal bins, cells counted blind to genotype, and then the relative number of BrdU positive cells calculated for each bin. Bin 10 represents the deepest bin, with bin 1 being closest to the cortical surface. d, h Statistical analysis revealed a significant difference when comparing +/Mbe heterozygotes with Mbe/Mbe mutants at E12.5 in bin 7 (n = 5 animals per genotype; two-way repeated measures ANOVA with Tukey’s multiple correction test; +/Mbe vs Mbe/Mbe P = 0.0242) and at E14.5 in bin 3 (n = 3 animals per genotype; two-way repeated measures ANOVA with Tukey’s multiple correction test; +/Mbe vs Mbe/Mbe P = 0.0465). At E14.5 Mbe/Mbe mutants have a higher percentage of Brdu positive cells in bin 3 indicative of a mild defect in neuronal migration. Unexpectedly, at E12.5 fewer cells are in bin 7, suggesting mildly faster migration. i-q Analysis of the cerebellum in +/+, +/Mbe, and Mbe/Mbe animals. Panels i-k show representative images of Nissl staining, l-n Foxp2 staining, and o-q Calbindin staining. The molecular layer, Purkinje cell layer, and granule cell layer all appear intact in Mbe/Mbe mutants (n = 3 animals per genotype). Error bars show mean +/- standard error of the mean. The scale bars in c and g show 200 μm and 150 μm in q.

Back to article page