Supplementary Figure 8: Simultaneous activation of 8 genes and 2 lncRNAs in mCherry-positive cells via hydrodynamic injection of all-in-one plasmids. | Nature Neuroscience

Supplementary Figure 8: Simultaneous activation of 8 genes and 2 lncRNAs in mCherry-positive cells via hydrodynamic injection of all-in-one plasmids.

From: In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice

Supplementary Figure 8

(a) Schematic of the all-in-one vector used for sgRNAs and Cre expression, and experimental flow of gene activations in the liver of SPH transgenic mice. Note that mRNA was extracted from mCherry-positive cells. (b) Simultaneous activation of 8 genes and 2 lncRNAs in the liver (n = 3 mice). The sgLacZ serves as a control sgRNA.

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