Supplementary Figure 2: Flow cytometry analysis of SPH-based activation. | Nature Neuroscience

Supplementary Figure 2: Flow cytometry analysis of SPH-based activation.

From: In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice

Supplementary Figure 2

(a) Schematic of the vectors used to evaluate SPH-based activation. Cre recombinase was used to remove the LSL cassette in the SPH vector. (b) Representative images show that co-transfection of sgRNA-miniCMV, SPH and Cre led to enhanced expression of mCherry (n = 4 cell cultures, 4 images). mCherry was activated in 93 ± 1% of the cells expressing SPH activator system. All values are presented as mean ± s.e.m.

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