Supplementary Figure 3: SPH shows more potent activation efficiency compared to SAM and VPR in primary astrocytes. | Nature Neuroscience

Supplementary Figure 3: SPH shows more potent activation efficiency compared to SAM and VPR in primary astrocytes.

From: In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice

Supplementary Figure 3

(a) Schematic illustration of all plasmids. The expressions of all functional elements were probed by the fluorescence reporter mCherry or GFP. (b) 2 days after transfection, cells were prepared for FACS. The mCherry and GFP double-positive cells were collected for qPCR. (c) SPH achieved the highest level of activation, which supports the observations in cell lines (n = 2 cell cultures).

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