Supplementary Figure 4: Characterization of SPH transgenic mice. | Nature Neuroscience

Supplementary Figure 4: Characterization of SPH transgenic mice.

From: In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR–dCas9-activator transgenic mice

Supplementary Figure 4

(a) Schematic of the integration sites of SPH in a SPH transgenic mouse genome. (b) Genotyping of SPH transgenic mice. “+” indicates positive mice. Percentage of positive progenies was calculated on 101 mice. Gel image was cropped. (c) Systemic evaluation of the Alb-Cre effectiveness in cleaving LSL cassettes specifically in the liver. Note that Alb is a liver-specific promoter (n = 1 mouse). Gel image was cropped. (d) Fluorescence images of the primary SPH fibroblasts infected by lentivirus containing sgRNA-EF1a-Cre-mCherry, confirming expression of dCas9 and activators were induced by Cre recombinase (n = 3 mice). (e) Expressions of dCas9 in Cre-expressing fibroblasts. Gel image was cropped. (f) Characterization of Cre effectiveness in the liver after hydrodynamic injection of the plasmids expressing Cre and mCherry (n = 1 mouse). (g) Stereotactic Injection of AAV-hSyn-mCherry-Cre to induce neuron-specific expression of EGFP (n = 1 mouse). Red arrowheads indicate primers. Scale Bars: d, f, g, 20 μm.

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