Supplementary Figure 4: 5-HT3AR+ interneurons provide spatially and temporally precise inhibition.
From: Inhibitory circuit gating of auditory critical-period plasticity
(a) Averaged responses (normalized ΔF/F) in primary auditory cortex (A1) to stimulation of ventral medial geniculate body (MGBv) using voltage-sensitive dye imaging (VSDI) within layers (L) 2/3, 4 and 5 of P12–15 mice (n = 10 mice) at baseline (black) or during 5-HT3AR cell optogenetic silencing (blue). (b) 5-HT3AR cell silencing decreased the early (0–40ms) peak response within L2/3/4 of activated columns (normalized ΔF/F: baseline = 0.68 ± 0.11; 5-HT3AR cell silencing = 0.49 ± 0.06, n = 10 mice; paired t test, two tailed, t(9) = −2.40, P = 0.0396, CL = −0.37, −0.01), but increased the late (200–450ms) response in neighboring columns (normalized ΔF/F: baseline = 0.51 ± 0.11; 5-HT3AR cell silencing = 0.68 ± 0.09, n = 10 mice; paired t test, two tailed, t(9) = 4.00, P = 0.0031, CL = 0.07, 0.27). Mean ± SEM, *P < 0.05, **P < 0.01.
