Supplementary Figure 9: Lynx1-knockout mice exhibit normal peripheral hearing thresholds.
From: Inhibitory circuit gating of auditory critical-period plasticity
(a, b) Representative auditory brainstem responses (ABR) recorded from C57 and Lynx1 KO adult mice during 11.3 kHz tone pips of increasing sound pressure level (decibels; dB). Circles represent location of the five positive (P) wave peaks (1–5). (c) C57 and Lynx1 KO mice showed similar ABR thresholds (mean ± SEM) across tone frequencies (MANOVA, ABR threshold changes by genotype, P = 0.51, frequency, P = 0.0018, genotype x frequency, P = 0.77). (d) C57 and Lynx1 KO mice showed similar Distortion Product Otoacoustic Emissions (DPOAE) thresholds (mean ± SEM) across tone frequencies (MANOVA, threshold changes by genotype, P = 0.10, frequency, P < 0.0001, genotype x frequency, P = 0.50, C57, n = 4 mice; Lynx1 KO, n = 6 mice). (e, f) Representative ABR waves recorded from Lynx1 KO adult mice before and 30 minutes after administration of DHβE (i.p. 1mg/kg) during 11.3 kHz tone pips of increasing dB. (g, h) DHβE treatment did not impact ABR or DPOAE thresholds (mean ± SEM) across tone frequencies (MANOVA, ABR threshold by treatment condition, P = 0.54, frequency, P = 0.0116, treatment x frequency, P = 0.98; DPOAE threshold by treatment condition, P = 0.26, frequency, P = 0.13, treatment × frequency, P = 0.70, n = 4 mice).
