Supplementary Figure 5: Photostimulation of somata and axon termini of CaMKIIα+ MPA neurons.
From: Medial preoptic circuit induces hunting-like actions to target objects and prey
a. Photostimulation of MPA neuron somata increases object dislocation by CaMKIIα::ChR2MPA mice (n = 6) more than control eYFPMPA mice (n = 7; Mann Whitney U test, Light on state between two groups, P = 0.008; Wilcoxon Signed Rank test, ON vs. OFF in ChR2, P = 0.031). b. Female CaMKIIα::ChR2MPA-vPAG mice (red lines; n = 4) moved presented objects more during photostimulation than control CaMKIIα::eYFPMPA-vPAG mice (gray lines; n = 5; Two Way RM ANOVA, ON state between two groups, P < 0.001; OFF vs. ON in ChR2, P < 0.001; ON vs. OFF in ChR2, P < 0.001). c. Bar graphs comparing object dislocation during photostimulation of CaMKIIα MPA somata in male mice and of the CaMKIIα MPA-vPAG circuit in male and female mice (n = 7 mice for MPA somata, n = 5 for CaMKIIα::ChR2MAP-vPAG male mice, n = 4 for CaMKIIα::ChR2MAP-vPAG female mice; One Way ANOVA, MPA somata vs. CaMKIIα::ChR2MAP-vPAG male, P < 0.001; CaMKIIα::ChR2MAP-vPAG male vs. CaMKIIα::ChR2MAP-vPAG female, P = 0.004). Line graphs represent mean (thick lines) ± SEM (error bars) and individual data (brighter lines). Bar graphs indicate mean (bars) ± SEM (error bars) and individual data (circles). See the detailed statistical values in Supplementary table 1. * P < 0.05
