Supplementary Figure 6: Attention shift to objects from females in the CaMKIIα::ChR2^MPA-vPAG mice.

a. Photostimulation elevates the locomotion of CaMKIIα::ChR2^MPA-vPAG (n = 8) mice but not in CaMKIIα::eYFP^MPA-vPAG (n = 5) mice (Unpaired t-test, P = 0.008). b. Representative behavioral raster plots for the inter-male aggression test. Each behavior is color-coded as indicated. 6 mice were used for this image. c. Percentage of social exploration and attacks versus non-social exploration behaviors (i.e., searching and digging) during encounters with an intruder male. Note that photostimulation reduced attacks toward the intruder male and increased non-social exploration. Photostimulation did not alter social exploration behavior (n = 6 for CaMKIIα::ChR2^MPA-vPAG mice, Paired t-test, social exploration, P = 0.308; attack, P = 0.003; non-social exploration, P = 0.007). Colored-boxes represent percentage of each behaviors. d. Duration of attacks by CaMKIIα::eYFP^MPA-vPAG (n = 6) and CaMKIIα::ChR2^MPA-vPAG (n = 6) mice (Mann Whitney U test, P = 0.026). e. Number of attacks by CaMKIIα::eYFP^MPA-vPAG (n = 6) and CaMKIIα::ChR2^MPA-vPAG (n = 6) mice (Unpaired t-test, P = 0.037). f. Percentage of time male CaMKIIα::ChR2^MPA-vPAG (n = 10) and CaMKIIα::eYFP^MPA-vPAG (n = 8) mice (Unpaired t-test, P = 0.00000415) spent searching for females during photostimulation. Note that, like what we observed in the inter-male aggression tests, CaMKIIα::ChR2^MPA-vPAG mice spent less time searching because they spent more time in non-social exploration. g. CaMKIIα::ChR2^MPA-vPAG (n = 6) eat less than CaMKIIα::eYFP^MPA-vPAG (n = 8) mice during photostimulation (Unpaired t-test, P = 0.001). Bar graphs show mean (bars) ± SEM (error bars) and individual data (circles). See the detailed statistical values in Supplementary table 1. * P < 0.05