Supplementary Figure 7.: In vivo activity of the CaMKIIα⁺ MPA–vPAG circuit measured by photometry.

a. The expression of GCAMP6m that mediated by viral infection of AAV9-CAG-DIO-GCaMP6m and AAV5-CaMKIIα::-Cre in MPA. 6 mice were used for these images. Scale bars, 200 µm. b. Representative raw trace of fluorescent activity [\(\Delta F/F=(F-{F}_{mean})/{F}_{mean}\)] during object exploration. c. Success rates, which indicate increase of fluorescent activity during object exploration, are not different across the bouts. (n = 6 mice; One Way RM ANOVA, P = 0.800) Bar indicate average of population and colored-lines indicate individuals. d. Peak fluorescent activity of individuals runs down across the bout number. (n = 6 mice) Bar graphs indicate mean (bars) ± SEM (error bars) and individual data (colored-lines). Dot graphs represent mean (dots) ± SEM (error bars), trend line (green line) and individual data (circles). See the detailed statistical values in Supplementary table 1. * P < 0.05