Supplementary Figure 3: Validation of virally mediated expression of ChR2 in VGLUT2::ChR2MPA-vPAG and VGAT::ChR2MPA-vPAG mice.
From: Medial preoptic circuit induces hunting-like actions to target objects and prey
a. Top, Schematic depiction of VGLUT2+ MPA-vPAG circuit photostimulation of VGLUT2-ires-Cre mice. Bottom, Representative images of virally mediated ChR2 expression in the MPA and its termini in vPAG. 5 mice were used for these images. b. Top, Schematic illustration of photostimulation in VGAT-ires-Cre mice. Bottom, Representative images of ChR2 expression in MPA and vPAG of VGAT-ires-Cre mice. 5 mice were used for these images. Scale bars, 200 µm
