Supplementary Figure 7: Microglia proliferate in the rNLS8 SC after hTDP43ΔNLS transgene suppression. | Nature Neuroscience

Supplementary Figure 7: Microglia proliferate in the rNLS8 SC after hTDP43ΔNLS transgene suppression.

From: Microglia-mediated recovery from ALS-relevant motor neuron degeneration in a mouse model of TDP-43 proteinopathy

Supplementary Figure 7

(a-c) Double-labeling of BrdU (white) and microglia (IBA-1+, red) in the SC of rNLS8 mice during disease (a) or early recovery (b-c) following injection of BrdU at 50 mg/kg, i.p. daily for 7 days from 6 weeks off DOX shows an increase in the number of newborn microglia in recovery. Though most of the BrdU-labeled cells were microglia, some were other cell types and these are indicated with yellow stars. Similar staining results were observed in n = 4 mice. Scale bar = 50 μm. (d) Quantitative analysis of BrdU-positive cells showing there was a trend towards increased BrdU+ cells in the ventral horn during early recovery when compared to disease rNLS8 mice, with most of these being co-positive for IBA-1. Data are mean ± S.D., n = 4 per group; unpaired t-test, t = 2.3, d.f. = 6, p = 0.07.

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