Supplementary Figure 1: When DOX is removed, there is neuron-specific hTDP-43 expression in rNLS8 mouse SC. | Nature Neuroscience

Supplementary Figure 1: When DOX is removed, there is neuron-specific hTDP-43 expression in rNLS8 mouse SC.

From: Microglia-mediated recovery from ALS-relevant motor neuron degeneration in a mouse model of TDP-43 proteinopathy

Supplementary Figure 1

(a) Representative image of microglia (green) and NEFH (blue) in a nTg mouse shows that NEFH is expressed in neurons, but not microglia with orthogonal views to show the lack of IBA-1 and NEFH co-localization. (b) Representative image of microglia (green) and hTDP-43 (blue) from rNLS8 SC after 3 weeks of hTDP43ΔNLS expression, with orthogonal views to show the lack of hTDP-43 localization. Scale bars in (a,b) = 25 μm. Similar staining was observed in 3 independent animals per genotype. (c) Representative images from 1 of 4 rNLS8 mice prior to DOX removal and after 1, 4, and 8 weeks off DOX stained with a marker for TDP-43 (red) that labels both mouse and hTDP-43. Note: nuclear clearance of TDP-43 in many neurons after DOX removal. Scale bar = 100 μm.

Back to article page