Supplementary Figure 3: Histone acetylation sites are identified on Grin2a and Grin2b promoters. | Nature Neuroscience

Supplementary Figure 3: Histone acetylation sites are identified on Grin2a and Grin2b promoters.

From: Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition

Supplementary Figure 3

(a) PCR images showing the ChIP (AcetyH3-occupied DNA), input (total DNA) and no-template control (NTC) signals with 3 primers (P1, P2, P3) designed against the promoter regions of Grin2a and Grin2b. The expected sizes of PCR products are labeled on the gels. The final primers used in the quantitative ChIP experiments are labeled by red circles. Top: diagram showing the primer locations on the 5′ upstream sequence of Grin2a and Grin2b. TSS, transcriptional start site. (b) The amplification curve and melt curve for AcetyH3-ChIP, input, and IgG control samples.

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