Supplementary Figure 2: Current antipsychotics fail to increase social interaction time, while the pan-HDAC inhibitor trichostatin A (TSA) transiently improves social behaviors in Shank3-deficient mice. | Nature Neuroscience

Supplementary Figure 2: Current antipsychotics fail to increase social interaction time, while the pan-HDAC inhibitor trichostatin A (TSA) transiently improves social behaviors in Shank3-deficient mice.

From: Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition

Supplementary Figure 2

(a–e) Box plots showing the time spent investigating either the social (Soc) or nonsocial (NS) stimulus during sociability testing in young (5-6 weeks old) male Shank3+/ΔC mice treated with fluoxetine (10 mg/kg, i.p., 14x, a, n=9), clozapine (5 mg/kg, i.p., 3x, b, n=11), valproic acid (VPA, 100 mg/kg, 3x, c, n=11), aripiprazole (1 mg/kg, 3x, d, n=9) or risperidone (0.1 mg/kg, 3x, e, n=10). In (c), F2,40(treatment)=0.71, P=0.5; +++ P<0.001 (Soc vs. NS), two-way rmANOVA. (f, g) Plots showing the preference index (f) and the time spent investigating either the Soc or NS stimulus (g) during sociability testing in Shank3+/ΔC mice before and after TSA treatment (0.5 mg/kg, i.p., 3x, n=8). In (f), F2,21=19.7, P<0.0001, one-way ANOVA. In (g), F2,28(treatment)=4.15, P=0.026; ++ P<0.01, +++ P<0.001 (Soc vs. NS), ### P<0.001 (pre- vs. post-injection), two-way rmANOVA. Inset (d,e,g): Representative heat maps illustrating the time spent in different locations of the 3 chambers (blue: 0 sec; red: ~20 sec) from the social preference tests of drug-treated Shank3+/ΔC mice.

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