Supplementary Figure 4: Additional behavioral outcomes from five-choice serial reaction time tasks and marble burying assays.
From: TDP-43 gains function due to perturbed autoregulation in a Tardbp knock-in mouse model of ALS-FTD
(a−b) Response latencies. Genotype comparison: P = 0.448 in 6−month−old; P = 0.181 12−month−old mice. (c−d) Reward collection latencies. Genotype comparison: P = 0.662 in 6−month−old; P = 0.821 12−month−old mice. (e−f) Premature responses. Genotype comparison: P = 0.068 in 6−month−old; P = 0.194 12−month−old mice. (g−h) Perseverative responses after correct response. Genotype comparison: P = 0.312 in 6−month−old; P = 0.291 12−month−old mice. At 6 months of age, n = 15 wild−type, 16 TDP−43Q331K/+, 15 TDP−43Q331K/Q331K mice, and at 12 months, n = 15 wild−type, 16 TDP−43Q331K/+, 16 TDP−43Q331K/Q331K mice. For (a−h) mixed−effects model was used and error bars denote s.e.m. (i) Correlation between omissions at 6 and 12 months of age in individual mice (n = 15 wild−type, 16 TDP−43Q331K/+, 15 TDP−43Q331K/Q331K mice). Comparisons: wild-type: P = 0.600 (ns); TDP−43Q331K/+: P = 0.392 (ns); TDP−43Q331K/Q331K: P = 0.046 (*); Pearson’s analysis. (j) Progressive marble burying of cohort 1 mice. Pairwise comparisons: 5 months (n = 19 wild−type, 19 TDP−43Q331K/+, 17 TDP−43Q331K/Q331K mice); wild−type vs. TDP−43Q331K/+: P = 0.03 (*); wild−type vs. TDP−43Q331K/Q331K: P = 0.013 (*); 8 months (n = 16 wild−type, 11 TDP−43Q331K/+, 15 TDP−43Q331K/Q331K mice); wild−type vs. TDP−43Q331K/+: P>0.999 (ns); wild−type vs. TDP−43Q331K/Q331K: P = 0.02 (*); 10 months (n = 16 wild−type, 14 TDP−43Q331K/+, 15 TDP−43Q331K/Q331K mice); wild−type vs. TDP−43Q331K/+: P = 0.03 (*); wild−type vs. TDP−43Q331K/Q331K: P = 0.03 (*); 12 months (n = 16 wild−type, 13 TDP−43Q331K/+, 15 TDP−43Q331K/Q331K mice); wild−type vs. TDP−43Q331K/+: P>0.999 (ns); wild−type vs. TDP−43Q331K/Q331K: P = 0.001 (**); 14 months (n = 15 wild−type, 13 TDP−43Q331K/+, 14 TDP−43Q331K/Q331K mice); wild−type vs. TDP−43Q331K/+: P = 0.396 (ns); wild−type vs. TDP−43Q331K/Q331K: P = 0.003 (**); 18 months (n = 15 wild−type, 13 TDP−43Q331K/+, 14 TDP−43Q331K/Q331K mice); wild−type vs. TDP−43Q331K/+: P = 0.009 (**); wild−type vs. TDP−43Q331K/Q331K: P < 0.0001 (****); Kruskal−Wallis followed by Dunn’s test for pairwise comparisons. Error bars for (j) represent median and interquartile range.
