Supplementary Figure 5: Frontal cortical histology.
From: TDP-43 gains function due to perturbed autoregulation in a Tardbp knock-in mouse model of ALS-FTD
(a) Nissl−stained coronal sections of frontal cortex. Representative images shown. Cortex scale bar, 1 mm; motor, cingulate and somatosensory cortex scale bars, 500 μm; motor cortex layer V scale bar, 500 μm.(b) Quantification of cells in cortical sub regions (n = 5 mice per genotype). Comparisons: Prefrontal: P = 0.477(ns); Motor: P = 0.931(ns); Motor layer V: P = 0.897(ns); Cingulate: P = 0.734(ns); Somatosensory: P = 0.150(ns); multiple t tests with correction using the Holm−Sidak method. (c) Quantification of frontal cortical area in TDP−43Q331K/Q331K mice relative to wild type (n = 6 mice per genotype). Comparison: P = 0.701 (ns); unpaired t test. Error bars denote s.e.m.(d) Uncropped western blots of frontal cortical tissue from four wild−type and four TDP−43Q331K/Q331K mice used in the quantification of TDP−43 in Fig. 4e.
