Supplementary Figure 3: Neuron and OPC numbers remain constant over the adult lifespan.
From: Myelin remodeling through experience-dependent oligodendrogenesis in the adult somatosensory cortex
a-c, Immunostaining of coronal sections somatosensory cortex from young (P60; a), middle-aged (P365; b), and aged (P665; c) Mobp-EGFP mice. Antibodies against EGFP, NeuN, and NG2 were used to label mature oligodendrocytes, neurons, and oligodendrocyte precursor cells (OPCs). d, Quantification of number of EGFP+ oligodendrocytes in a 0.6 mm2 area over age (n = 4 mice per group; one-way ANOVA with Tukey's posthoc test, young vs. middle, P = 0.004, q(11) = 6.44; young vs. aged, P = 0.003, q(11) = 6.56; middle vs. aged, P = 0.90, q(11) = 0.12; n.s. = not significant; * = P < 0.005). e, Quantification of number of NeuN+ neurons in a 0.6 mm2 area over age (n = 4 mice each group; one-way ANOVA P = 0.44, F(2, 9) = 0.90; n.s. = not significant). f, Quantification of oligodendrocyte precursor cell number in a 0.6 mm2 area in young, middle-aged and aged mice (n = 4 mice each group; P = 0.95, F(2, 9) = 0.05, one-way ANOVA; n.s. = not significant). d-f, Data is presented as mean ± SEM.
