Supplementary Figure 4: Specificity of expression in fate-mapped mouse OPCs.
From: Myelin remodeling through experience-dependent oligodendrogenesis in the adult somatosensory cortex
Maximal projections from triple transgenic mice (Olig2-CreER; R26-lsl-tdTomato;NG2-mEGFP) at P150 (100 mg/kg tamoxifen injected for five days at P30, n = 3 mice) immunostained for EGFP, tdTomato and a marker of mature oligodendrocytes or OPCs. a, A subset of mature oligodendrocytes (ASPA+) express tdTomato. b, OPCs that express membrane-anchored EGFP and tdTomato were immunoreactive for PDGF receptor alpha (PDGFRa). Note mature oligodendrocyte that does not express EGFP and is not labeled with PDGF receptor alpha (yellow arrowhead). c, A small population of astrocytes are recombined and express tdTomato only. Astrocytes are not immunolabeled with PDGF receptor alpha and are distinguished by their distinct bushy morphology. Note the OPC labeled with PDGF receptor alpha that expresses both EGFP and tdTomato (yellow arrowhead).
