Supplementary Figure 10: Cdh8 and Sorcs3 loss-of-function mutations.
From: Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
(a) Cross-sections of OFF alpha RGCs labeled by TYW7; Cdh8+/lacZ and TYW7; Cdh8lacZ/lacZ retinas. (b) Distribution of GFP intensities from OFF alpha RGC dendrites along IPL depth. n=10 and 8 sections from Cdh8+/lacZ and Cdh8lacZ/lacZ animals respectively, 3 animals per genotype. p=0.12, Cochran-Armitage test. Scale=25µm. Lines and brackets indicate mean and standard error. (c) DNA construct for AAV encoding short-hairpin RNA against Sorcs3 (shSorcs3). (d-e) Whole mount view of GCL of a retina infected with control AAV expressing Td-Tomato (TdT), or AAV encoding shSORCS3. Sorcs3 protein is greatly reduced in shSORCS3-treated retinas. Scale=25µm. Each experiment in d-e was repeated independently in 3 and 5 animals respectively with similar results.
