Supplementary Figure 6: In situ mRNA staining using RNAScope (related to Fig. 5).

a) Cervical and lumbar section stained for Nmu (red), Tac2 (green) and Sst (blue) in order to detect cells representing the clusters Glut5-7. The celltypes are highlighted in the small panels on the right using arrows (Glut5 [red arrow], Glut6 [yellow arrow], Glut7 [white arrow]). The small panels represent the magnified region depicted by the yellow frame in the corresponding panel on the left. b) Cervical and lumbar section stained for Qrfpr (red), Crabp1 (green) and Npy (blue) in order to detect cells representing the clusters Gaba5-7. The cell types are highlighted in the small panels on the right using arrows (Gaba5 [red arrow], Gaba6 [yellow arrow], Gaba7 [white arrow]). The small panels represent the magnified region depicted by the yellow frame in the corresponding panel on the left. c) Two example cells for cluster Gaba1 expressing beside Gal both Slc17a6 and Gad1. d) Images of the lumbar and cervical section that were used as reference sections to the Allan Brain Reference Atlas. e) In order to align two sections, we defined in total 10 Reference points for each section (including the reference section; 5 for the left and right horn respectively, starting always at the central canal). Each image was then cropped and transformed so that they could be aligned using the reference points. Scale bars = 200 µm for overview picture and 50 µm for magnifications in panels a) and b), 20 µm in panel c) as well as 500 µm in panel d) and e).