Supplementary Figure 3: Relation of the identified neuron types to known literature (related to Figs. 1, 2 and 3).

a) Neurochemistry and morphology. Green indicates glutamatergic neurons, red GABAergic neurons. The lines depict predicted detection of the markers using immunohistochemistry. Immunohistochemistry is not as sensitive or quantitative as in situ hybridization. This figure therefore only depicts the high-expressing cell-types that we predict have been detected in the cited studies. The actual expression data of these markers is available as Supplementary Fig 4a. For morphology, CALB2⁺ and PVALB⁺ cells are often islet cells, PDYN⁺ and NPY⁺ are heterogenous but never Islet cells, PDYN⁺ cells are sometimes vertical cells. b) Predicted function of neuron types. Illustration of neuron types subjectively predicted to have been functionally analyzed. Note that mouse models used Cre driver lines and thus cells expressing the gene at any point in development will recombine and be included in the study and therefore, this summary has to be interpreted with this in mind. Due to transient developmental expression or low levels of expression, studies on Slc17a8, Ret, Bhlhb5, Slc6a5 were excluded. Stars indicate the gene also to be expressed in neurons belonging to the other neurotransmitter type which is not shown in the figure. For the actual expression profile of these genes in both neurotransmitter types, see Supplementary Fig. 4a.