Supplementary Figure 5: Analgesic tolerance to κ-opioid agonist, U62,066, and AC activities in the RVM. | Nature Neuroscience

Supplementary Figure 5: Analgesic tolerance to κ-opioid agonist, U62,066, and AC activities in the RVM.

From: Complex formation between the vasopressin 1b receptor, β-arrestin-2, and the μ-opioid receptor underlies morphine tolerance

Supplementary Figure 5

(A) WT and V1b-/- mice were injected with U62,066 (10 mg/kg i.p.) once a day for 12 days. Tail flick latencies were examined 30 min after the injections. The basal latencies on day 1 prior to drug injection were 5.0 ± 0.5 and 8.2 ± 1.0 sec for WT (n = 12) and V1b-/- (n = 11) mice, respectively (unpaired, two-sided Student’s t-test, t15 = 2.7281, P = 0.0154). Treatment periods had significant influence on tail-flick latencies (F11,251 = 6.133, P < 0.0001), but genotype did not (F1,251 = 1.584, P = 0.209) in two-way ANOVA analysis. (B) Tolerance to U62,066 had no effect on AC activity in the RVM of WT and V1b-/- mice. n = 7,7 WT mice and n = 10, 13 V1b-/- mice for saline and U62,066 treatments. Graphs represent the mean ± S.E.M.

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