Supplementary Figure 6: Radioligand binding studies for examining functional interactions between AVP and opioid agonists. | Nature Neuroscience

Supplementary Figure 6: Radioligand binding studies for examining functional interactions between AVP and opioid agonists.

From: Complex formation between the vasopressin 1b receptor, β-arrestin-2, and the μ-opioid receptor underlies morphine tolerance

Supplementary Figure 6

[3H]naloxone (1 nM) was incubated with membrane samples from HEK-μ-receptor cells (A) or from HEK-V1bR-μ-receptor cells (B) in increasing concentrations of morphine (A) or DAMGO (B) in the presence or absence of 100 nM AVP. (C) In saturation binding experiments on HEK-V1bR- μ-receptor cells, increasing concentrations of [3H]AVP (0 – 30 nM) were incubated with membrane samples in the presence of saline or 1 μM morphine. Data shown in A–C are means from four (A and B) or three (C) independent experiments. Graphs represent the mean ± S.E.M.

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