Supplementary Figure 1: Binding sites and expression profile of V1bR in mice. | Nature Neuroscience

Supplementary Figure 1: Binding sites and expression profile of V1bR in mice.

From: Complex formation between the vasopressin 1b receptor, β-arrestin-2, and the μ-opioid receptor underlies morphine tolerance

Supplementary Figure 1

(A) Membrane fractions (0.2 mg/reaction) from whole brains of WT (n = 5) and V1b-/- (n = 3) mice were subjected to a saturation binding study using the μ-receptor-specific radioligand, [3H]DAMGO. Bmax values were 114 ± 6 and 95 ± 5 fmol/mg protein for WT (n = 5) and V1b-/- (n = 3), respectively; unpaired two-sided Wilcoxon rank sum test, P = 0.114. KD values are 0.8 ± 0.2 and 0.9 ± 0.1 nM for WT and V1b-/- mice, respectively. (B) V1bR transcripts were detected in the RVM (gray bar), but were scarce in the dorsal medulla, spinal cord and dorsal root ganglia. For positive control, pituitary tissue was included (C). The expression levels of V1bR transcripts were normalized against GAPDH (n = 5). Graphs represent the mean ± S.E.M.

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