Supplementary Figure 6: CAMK2A-expressing CRH neurons in the aBNST and CeA project to the VTA. | Nature Neuroscience

Supplementary Figure 6: CAMK2A-expressing CRH neurons in the aBNST and CeA project to the VTA.

From: Chronic CRH depletion from GABAergic, long-range projection neurons in the extended amygdala reduces dopamine release and increases anxiety

Supplementary Figure 6

Representative confocal images of Crh-ires-Cre mice injected with AAV-Camk2a::DIO-EYFP into the aBNST (a) or CeA (b). EYFP expression is driven by the Camk2a promoter specifically in CRH neurons. EYFP-labeled projections that originate from CAMK2A-expressing neurons of the aBNST or CeA (top panels) are detected in the VTA and SNc (bottom panels). Bottom right depict higher magnification images of the VTA/SN midbrain region. Representative images were derived from three independent experiments each. Abbreviations: anterior commissure (ac), anterior dorsal BNST (adBNST), basolateral amygdala (BLA), central amygdala (CeA), periaqueductal gray (PAG), substantia nigra compacta (SNc), substantia nigra reticularis (SNr), ventral tegmental area (VTA). Scale bars represent 100 µm.

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