Supplementary Figure 3: Isolation of interneurons migrating through the MZ and SVZ.
From: Optimization of interneuron function by direct coupling of cell migration and axonal targeting
(a) Schematic of experimental design for the isolation of neurons in progressively deeper positions of the embryonic cortex. (b) Boxplots represent median, 1st and 3rd quartile, and 1.5 IQR for percentage of TdTomato (CR + cells) and Tbr2 + cells in each fraction, normalized to total cell number, n = 3 litters of embryos. (c–g) Representative images showing TdTomato (CR + cells) and Tbr2 + cells in the different cellular fractions after dissociation and plating. Fraction 1 is closest to the pial surface and fraction 5 is closest to the ventricular zone (VZ). Experiments were repeated 3 times with similar results. Scale bar equals 200 µm.
