Supplementary Figure 11: Intrinsic properties of Martinotti cells in Mafb conditional mutants.
From: Optimization of interneuron function by direct coupling of cell migration and axonal targeting
(a) Representative traces of voltage response to a depolarizing 150 pA current injection in layer 2/3 Martinotti cells from P21-28 SstCre;Mafbfl/+;RCE and SstCre;Mafbfl/fl;RCE mice. (b) Table showing intrinsic properties of layer 2/3 Martinotti cells from P21-28 SstCre;Mafbfl/+;RCE and SstCre;Mafbfl/fl;RCE mice. p-values calculated by two-tailed Mann-Whitney U test. Bar graph represents mean ± s.e.m., n = 22 and 16 cells, from 10 Mafbfl/+;RCE and10 SstCre;Mafbfl/fl;RCE mice, respectively. (c-g) Quantification of resting membrane potential (c), input resistance (d), action potential threshold (e), rheobase (f) and action potential half width (g). for (c-g), Bar graph represents mean ± s.e.m., n = 22 and 16 cells, from 10 Mafbfl/+;RCE and10 SstCre;Mafbfl/fl;RCE mice, respectively. (h) Number of action potentials evoked in response to increasing current injections in layer 2/3 Martinotti cells from P21-28 SstCre;Mafbfl/+;RCE and SstCre;Mafbfl/fl;RCE mice. Data is presented as mean ± s.e.m., n = 22 and 16 cells, from 10 MafBfl/+;RCE and10 SstCre;Mafbfl/fl;RCE mice, respectively (i) Bar graph represents mean ± s.e.m. of maximum action potential frequency evoked in layer 2/3 Martinotti cells from P21-28,n = 22 and 16 cells, from 10 Mafbfl/+;RCE and10 SstCre;Mafbfl/fl;RCE mice, respectively; two-tailed Student's T-test p = 0.30. (j) Fraction of the maximal response recorded from individual layer 2/3 pyramidal neurons following stimulation of ChR2-expressing SST + cells in SstCre;Mafbfl/+;RCE and SstCre;Mafbfl/fl;RCE mice at increasing LED intensities. Data is presented as mean ± s.e.m., n = 19 and 12 cells 6 SstCre;Mafbfl/+;RCE and 7 SstCre;Mafbfl/fl;RCE mice, respectively. (k) Bar graph represents mean ± s.e.m. for of the minimal LED intensity required to evoke a detectable input in layer 2/3 pyramidal neurons from SST + cells expressing ChR2, n = 19 and 12 cells from 6 SstCre;Mafbfl/+;RCE and 7 SstCre;Mafbfl/fl;RCE mice, respectively. Two-tailed Student's T-test, p = 0.39. RMP, resting membrane potential; Capacitance, membrane capacitance; IR, input resistance; Sag, h-current sag; AP Threshold, action potential threshold; Amplitude, action potential amplitude; Rise time, action potential rise time; Decay time, action potential decay time; Half width, action potential half width; fAHP, fast after-hyperpolarization amplitude; Delay, delay to first action potential.
