Supplementary Figure 2: Early commitment to migratory routes by MGE-derived interneurons.
From: Optimization of interneuron function by direct coupling of cell migration and axonal targeting
(a) Schematic of experimental design for the imaging of Kikume Green-Red labeled interneurons. (b,c) Time-lapse images of Kikume Green-Red labeled interneurons immediately after photoconversion (b) and at the end of the experiment (c). The arrowhead indicates a cell migrating out of the MZ. Experiments were repeated over 10 different slices with similar results. (d) Cumulative frequency of migration speed for interneurons in the MZ (n = 48 cells). The dotted line represents the theoretical minimum speed required for migrating interneurons to leave the MZ during the experimental time (14 h). (e) Quantification of the fraction of cells at the MZ; n = 48 (theoretical), n = 141 (photo-converted) cells from 10 different slices; two-tailed Fisher's exact test, ***p < 0.001 (p = 7.03 e-8). (f) Schematic of experimental design for slice transplantation experiments. (g,h) Representative examples of slices following transplantation of MZ (g) and SVZ (h) derived interneurons. Asterisks indicate the original placement of transplanted cells. (i) Boxplots represent median, 1st and 3rd quartile, and 1.5 IQR for fraction of cells located in the MZ following transplantation of MZ and SVZ-derived interneurons. n = 10 and12 slices, from 5 and 6 animals for MZ and SVZ derived cells respectively; two-tailed Student t-test, **p < 0.01 (p = 4.545 e-5). Scale bars equal 100 µm (b,c) and 200 µm (g,h).
