Supplementary Figure 2: Thorny and athorny neurons can be labeled by Golgi staining.

a) Schematic of hippocampal region isolated for Golgi staining. b) Low magnification bright-field image of Golgi stained hippocampus (opaque, black) and co-staining with cresyl violet to indicate the locations of cell bodies (n = 3 animals). c) Representative example of a thorny pyramidal cell which were abundant in each brain tested, where thorny excrescences adorn the proximal apical dendrite indicated by red arrows (n = 5 cells). d) Representative example of an athorny cell which were reliably identified in each brain tested, where no complex multi-headed spines are apparent along the main apical dendritic shaft although simple spines are present, indicating that the imaged cell is unlikely to be an inhibitory interneuron (n = 10 cells identified).