Supplementary Figure 4: Adult locomotion and anxiety-like behavior are not affected by cross-fostering, cesarean delivery, and inoculation of maternal vaginal fluid.

(a-c) No difference in transitions, time in light, and distance travelled in the light-dark box paradigm between vaginally delivered control and prenatal stress-exposed adult males, or between C→C, C→S, S→C, and S→S adult males. Vaginally delivered control and prenatal stress-exposed males were tested separately from C→C, C→S, S→C, and S→S males (Vaginally delivered control vs. prenatal stress exposed males, transitions, t₁₂ = 1.591, P = 0.1376; time in light, t₁₂ = 0.0019, P = 0.999; distance, t₁₂ = 0.37, P = 0.7177, Unpaired two-sided t-Test; Comparison between C→C, C→S, S→C, and S→S males, transitions, F_3,12 =0.106, P = 0.95; time in light, F_3,12 =0.4836, P = 0.701; distance, F_3,12 =0.2604, P = 0.8523, One-way ANOVA). Box-whisker plot center line, median; box limits, upper and lower quartiles; whiskers represent Tukey fences. N = 7 vaginally delivered control males; N = 7 vaginally delivered prenatal-stressed males; N = 4 C→C males; N = 3 C→S males; N = 6 S→C males; N = 3 S→S males. Data represented as box-whisker plot. (d-f) No difference in transitions, time in light, and distance travelled in the light-dark box paradigm between vaginally delivered control and prenatal stress-exposed adult females, or between C→C, C→S, S→C, and S→S adult females. Vaginally delivered control and prenatal stress-exposed females were tested separately from C→C, C→S, S→C, and S→S females. (Vaginally delivered control vs. prenatal stress exposed females, transitions, t₁₂ = 0.5078, P = 0.6208; time in light, t₁₂ = 1.025, P = 0.3255; distance, t₁₂ = 0.0448, P = 0.965, Unpaired two-sided t-Test; Comparison between C→C, C→S, S→C, and S→S females, transitions, F_3,8 =0.7957, P = 0.5299; time in light, F_3,8 =0.7363, P = 0.5592; distance, F_3,8 =0.3216, P = 0.81, One-way ANOVA). Box-whisker plot center line, median; box limits, upper and lower quartiles; whiskers represent Tukey fences. N = 7 vaginally delivered control females; N = 7 vaginally delivered prenatal stress-exposed females; N = 3 C→C females; N = 3 C→S females; N = 3 S→C females; N = 3 S→S females. Data represented as box-whisker plot.