Supplementary Figure 10: Exposure to dying cells triggers the expression of the cbMg-enriched clearance genes in forebrain microglia in vitro.
From: Epigenetic regulation of brain region-specific microglia clearance activity
(a) Schematic showing exposure of primary microglia to early apoptotic cells in vitro. (b-g) Dot plots show relative expression of selected cbMg-enriched clearance-associated, stMg-enriched surveillance-associated, or pro-inflammatory genes in primary microglia exposed to vehicle or early apoptotic cells. (b) Anxa2 (4hr: p<0.0001, t4=11.13; 12hr: p<0.0001, t4=36.96), Colec12 (4hr: p=0.072, t4=2.433; 12hr: p=0.002, t4=7.498), Lilrb4 (4hr: p=0.019, t4=3.799; 12hr: p<0.0001, t4=14.04), Apoe (4hr: p=0.926, t4=0.09957; 12hr:p=0.002, t4=6.964), Cd74 (4hr: p=0.146, t4=1.801; 12hr: p=0.001, t4=8.070), Ptch1 (4hr:p=0.008, t4=4.920; 12hr: p=0.037, t4=3.066), Clec7a (4hr: p=0.001, t4=9.045; 12hr: p=0.015, t4=4.064), Msr1 (4hr: p=0.436, t4=0.8643; 12hr: p=0.011, t4=4.448), Lyz2 (4hr: p=0.092, t4=2.203; 12hr: p=0.024, t4=3.529), Ptplad2 (4hr: p=0.004, t4=6.109; 12hr: p=0.009, t4=4.809). (c) Pparg (4hr: p=0.916, t4=0.1120; 12hr: p=0.002, t4=7.144), Jdp2 (4hr: p<0.0001, t4=16.66; 12hr: p<0.0001, t4=15.16), Rarg (4hr: p<0.0001, t4=20.03; 12hr: p=0.001, t4=10.16), Tfec (4hr: p=0.002, t4=7.261; 12hr: p<0.0001, t4=11.47); Ahr (4hr: p<0.0001, t4=14.08; 12hr: p=0.004, t4=6.033), En2 (4hr: p=0.468, t4=0.8004; 12hr: p=0.025, t4=3.493), Tead4 (4hr: p=0.624, t4=0.5296; 12hr: p=0.002, t4=7.013). (d) Slc2a5 (4hr: p=0.088, t4=2.496; 12hr: p<0.0001, t4=15.30), Asb2 (4hr: p<0.0001, t4=13.82; 12hr: p<0.0001, t4=25.35), Tmem119 (4hr: p=0.001, t4=9.393; 12hr: p=0.003, t4=6.721), Fscn1 (4hr: p=0.501, t4=0.7381; 12hr: p<0.0001, t4=18.93), P2ry12 (4hr: p=0.175, t4=1.647; 12hr: p=0.733, t4=0.3659), Fcrls (4hr: p=0.043, t4=2.937; 12hr: p=0.001, t4=10.31). (e) Hhex (4hr: p=0.961, t4=0.05233; 12hr: p=0.025, t4=3.501), Esr1 (4hr: p=0.362, t4=1.029; 12hr: p=0.018, t4=3.885), Irf8 (4hr: p=0.023, t4=3.610; 12hr: p=0.001, t4=9.580), Sall1 (4hr: p=0.034, t4=3.163; 12hr: p=0.139, t4=1.842), Sall3 (4hr: p=0.695, t4=0.4223; 12hr: p=0.449, t4=0.8381). (f) Ccl2 (4hr: p=0.002, t4=7.739; 12hr: p=0.022, t4=3.632), Cxcl10 (4hr: p=0.218, t4=1.459; 12hr: p=0.020, t4=3.757), Mx1 (4hr: p=0.609, t4=0.5541; 12hr: p=0.879, t4=0.1659), Tnf (4hr: p=0.175, t4=1.648; 12hr: p=0.462, t4=0.8128), Ifnb1 (4hr: p=0.475, t4=0.7882; 12hr: p=0.864, t4=0.1830). (g) Kdm6b (4hr: p=0.005, t4=5.645; 12hr: p<0.0001, t4=15.33), and Kdm6a (4hr: p=0.524, t4=0.6979; 12hr: p=0.004, t4=5.871). Dot plots show mean ± SEM. Unpaired two-tailed t-test.
