Supplementary Figure 5: Crf- and Cck-BNST neurons: anxiety-like behavior. | Nature Neuroscience

Supplementary Figure 5: Crf- and Cck-BNST neurons: anxiety-like behavior.

From: Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states

Supplementary Figure 5

(a) Velocity and (b) percent time spent in the center in the open field test following Crf-BNST DREADD manipulation (n=19 mCherry, n=9 hM3Dq, n=7 hM4Di, two-way RM-ANOVA; DREADD x CNO interaction for velocity: F2,32 = 1.86, p = 0.172, DREADD x CNO interaction for center time: F2,32 = 6.03, p = 0.006, Bonferroni post-hoc comparisons *p < 0.05 vs. saline control). Centre and error bars are mean ± S.E.M. (c) Velocity and (d) percent time spent in the center in the open field test following Cck-BNST DREADD manipulation (n=15 mCherry, n=11 hM3Dq, n=4 hM4Di, two-way RM-ANOVA; DREADD x CNO interaction for velocity: F2,28 = 5.52, p = 0.010, Bonferroni post-hoc comparisons **p < 0.005 vs. saline control, DREADD x CNO interaction for center time: F2,28 = 4.99, p = 0.014, Bonferroni post-hoc comparisons *p < 0.05 vs. saline control). Centre and error bars are mean ± S.E.M. (e) Percent open arm time in the elevated plus maze (EPM) following Crf-BNST DREADD manipulation (n=21 mCherry, n=21 hM3Dq, n=13 hM4Di, two-way RM-ANOVA; DREADD x CNO interaction: F2,52 = 14.51, p < 0.0001, Bonferroni post-hoc comparisons ***p < 0.0001 vs. saline control). Centre and error bars are mean ± S.E.M. (f) Crf-BNST-ChR2 10Hz optogenetic stimulation increased anxiety-like behavior (n=11 mice, two-tailed paired t-test; t10 = 4.16, **p = 0.0020 vs. no-stimulation control). Centre and error bars are mean ± S.E.M. (g) Percent open arm time in the EPM following Cck-BNST DREADD manipulation (n=16 mCherry, n=11 hM3Dq, n=10 hM4Di, two-way RM-ANOVA; DREADD x CNO interaction: F2,34 = 9.431, p = 0.0006, Bonferroni post-hoc comparisons ***p < 0.0001 vs. saline control. Centre and error bars are mean ± S.E.M. (h) Cck-BNST-ChR2 10Hz optogenetic stimulation increased anxiety-like behavior (n=12 mice, two-tailed paired t-test; t11 = 5.53, ***p < 0.0005 vs. no-stimulation control). Centre and error bars are mean ± S.E.M.

Back to article page