Supplementary Figure 3: Crf and Cck: non-overlapping BNST subpopulations, differential inputs onto LH neurons. | Nature Neuroscience

Supplementary Figure 3: Crf and Cck: non-overlapping BNST subpopulations, differential inputs onto LH neurons.

From: Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states

Supplementary Figure 3

(a) Genetic screen of Cre-inducible Ai14 (tdTomato) reporter expression in adBNST neurons of wild-type mice, and mice from seven different BNST marker Cre driver lines, replicated independently with similar results in three mice. (b-c) Representative images of CRF and CCK immunostaining (red) in adBNST and pBNST and slices from LepRb-LH-RVdG-GFP mice, replicated independently with similar results in three mice. White arrowheads indicate neuropeptide co-expressing input cells. (d) Representative co-expression between Crf-Cre, Cck-Cre, Vgat-Cre, or Vglut2-Cre Ai14 (tdTomato, red) reporter expression and CRF or CCK (green) immunostaining in adBNST neurons, replicated independently with similar results in three mice. (e) Representative co-expression between CRF immunostaining & Drd1-Ai14 (top), and Tac1-Ai14 (bottom) in adBNST neurons, replicated independently with similar results in three mice. Scalebars: all 100 μm.

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