Supplementary Figure 3: Visualizing and quantifying urination behavior.
From: Voluntary urination control by brainstem neurons that relax the urethral sphincter
a, Schematic of behavioral setup for simultaneous optogenetics/fiber photometry, video recording, and analysis of urine excretion of awake behaving mice. b, Left: top camera records mouse position, right: urine fluoresces under UV light enabling excretion to be visualized throughout assay. Grey carrot indicates position between synchronized images from top and bottom cameras. c, Example of automated urine pixel detection with calibration data consisting of 4 replicates of 8 different volumes of male mouse urine on thin chromatography paper. d, Second order polynomial fit to calibration data on thick and thin paper; coefficients from these were used to calculate all urine amounts reported in microliters.
