Supplementary Figure 4: Whole-cell recordings of Bar^CRH-ChR2 and Bar^ESR1-ChR2 neurons during photostimulation.

a, Example current clamp traces from representative Bar^CRH-ChR2 (magenta), Bar^ESR1-ChR2 (green), and Bar^ESR1-GFP (orange) neurons during 5 sec photostimulation bouts at 10/25/50 Hz. b, Visualization of recording location of Bar^ESR1-ChR2 neuron in (a) showing ChR2-tdTomato expression. c, Stimulated firing rates (each circle represents the mean firing rate during the stimulation period for an individual neuron) versus photostimulation frequency for all recorded Bar^CRH-ChR2 (magenta, n = 6), Bar^ESR1-ChR2 (green, n = 12), and Bar^ESR1-GFP (orange, n = 4) neurons. Most neurons, particularly Bar^CRH-ChR2, are affected by depolarization block at 50 Hz. Scale bars = 100 μm.