Supplementary Figure 5: Frequency characteristics of urethral sphincter bursting during natural behavior and after Bar photostimulation.

a, Schematic for optogenetic Bar stimulation during EUS EMG recording. b, Example raw EUS EMG and corresponding spectral power in the 5-15 Hz band for photostimulated burst responses in Bar^CRH-ChR2 (top) and Bar^ESR1-ChR2 (bottom) mice. Bar^CRH-ChR2 burst is preceded by an increase in tonic activity during the photostimulation period, whereas Bar^ESR1-ChR2 burst occurs at low latency without preceding tonic activity, and displays decreasing frequency characteristic of natural bursts in (f). c, Example video frame from wireless corpus spongiosum pressure recording in the presence of female odor (yellow shading). d, Schematic of corpus spongiosum recording setup. e, Corpus spongiosum pressure recording after presentation of female odor. Top, raw pressure; bottom, spectral power in the 5-15 Hz band. Yellow arrows mark approximate start times for urination events. f, Shorter timescale recordings as in (e), for 5 urination events across 2 mice. Binary images on the left show relative sizes of thresholded urine marks corresponding to bursts on the right. Frequency typically decreases over a large burst lasting a few seconds, although shorter bursts with less urine were also observed (2^nd from top).