Supplementary Figure 1: Neurotransmitter identity and direct spinal projections of BarESR1 neurons.
From: Voluntary urination control by brainstem neurons that relax the urethral sphincter
a, Anti-ESR1 overlap with ESR1-ZsGreen (Ai6) genetic reporter. Bottom is larger view without Nissl. b, Anti-ESR1 overlap with Vglut2-ZsGreen genetic reporter. c, Anti-ESR1 overlap with Vgat-ZsGreen genetic reporter. d, RnaScope in-situ hybridization of Crh/Esr1/Vglut2 mRNA in Bar region of a wild-type male mouse, 20X objective. e, Larger view of dotted area in (d), 40X objective. f, g, Close-up views of individual cells in (e), with DAPI counterstain. h-k, same as (d)-(g), but with Vgat mRNA probe. l, Schematic of CTB injection into S1 spinal cord. m, CTB injection site. n, Retrograde CTB labeling in Bar with anti-ESR1 and CRH-tdT. Dotted ovals delineate Bar. Scale bars = 100 μm, except panels f/g/j/k, scale bars = 20 μm.
