Supplementary Figure 6: Multiplex FISH validation of rosehip marker co-expression in parietal (Brodmann Area 40) and frontal (Brodmann Area 9) regions of cortex.
From: Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type
A-F, Three distinct gene combinations were run in each brain region. Arrowheads show examples of RCs located in layer 1 of each brain region based on marker gene expression identified from RNA-Seq data. RCs were double positive for the marker genes shown and were either negative for CNR1 or had very low levels of CNR1 (C, E) in comparison to adjacent CNR1-positive cells (C). Multiplex FISH experiments were conducted on n=2 tissue donors.
