Supplementary Figure 1: Gating strategy for collection of single nuclei.
From: Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type
A, Nuclei were first gated based on size (forward scatter area, FSC-A) and granularity (side scatter area, SSC-A). B, Nuclei were then gated on DAPI fluorescence, followed by gates to exclude doublets and aggregates (C – FSC-single cells [SC], D – SSC-SC). E, Lastly, nuclei were gated based on NeuN Alexa Fluor 594 signal (NeuN-AF594-A) to differentiate neuronal (NeuN+) and non-neuronal (NeuN-) nuclei.
