Supplementary Figure 2: Marker gene expression patterns across identified single nuclei clusters.
From: Transcriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type
A, Heatmap of log-normalized expression (CPM) in single nuclei grouped by clusters that have been ordered by transcriptomic similarity. Canonical gene markers (SNAP25, GAD1, SLC17A7) classify clusters into broad classes of excitatory and inhibitory neuron and non-neuronal cell types. Within these broad types, many genes discretely mark individual clusters. B, Colorimetric ISH in mouse cortex of marker genes shown for human temporal cortex in Figure 1. Red arrows highlight cells with expression in layer 1. ISH experiments were repeated on multiple tissue specimens as follows: Sst, Lhx6,Ndnf (n=2); Slc17a7, Vip, Sema3c (n=3), Cck (n=4); Reln, Cnr1 (n=5); Gad1, Pvalb (n=20).
