Supplementary Figure 8: Absence of reporter protein expression in phagocytes isolated from blood and lymph nodes of iNOS-tdTomato-Cre × Arginase-YFP mice, iNOS-tdTomato-Cre × Rosa26-Stp-fl-YFP and Rosa26-Stp-fl-YFP mice.
From: Mononuclear phagocytes locally specify and adapt their phenotype in a multiple sclerosis model
(a) Flow cytometric analysis of blood isolated from iNOS-tdTomato-cre x Arginase-YFP mice at peak of EAE. Populations were gated based on expression of CD45 and CD11b. Average percentage of polarized cells ( ± s.e.m.) are displayed, n = 5 mice. (b) Flow cytometric analysis of lymph node preparation from iNOS-tdTomato-cre x Arginase-YFP mice at peak of EAE. Populations were gated based on their expression of CD45 and CD11b. Average percentage of YFP+ and/or tdTomato+ cells ( ± s.e.m.) are displayed. Analysis based on 3 independent experiments with a total of 8 mice. (c,d) Flow cytometric analysis of cells isolated from the blood of iNOS-tdTomato-cre x Rosa26-Stp-fl-YFP (c, n = 5 mice) and control Rosa26-Stp-fl-YFP (d, n = 8 mice) at peak of EAE. Populations were gated based on expression of CD45 and CD11b. Average percentage of YFP+ and/or tdTomato+ cells ( ± s.e.m.) are displayed.
