Supplementary Figure 7: Neural activities in M1 L5 neurons increase during territorial marking.
a, Heatmap of the Ca2+ signals was merged with spatial distribution of urine spots (ovals) and with positions when the mouse was still (triangles). The mouse was tested in a home cage lined with filter paper over 5 days (2 h for each day). The mouse was not given saline intraperitoneal (i.p.) injection before testing. b, Comparison of the amplitudes of Ca2+ signals during urinationand when the animals were still. One-sided Wilcoxon rank-sum test, ***P = 4.5 × 10–6 (z = 4.438; df = 28); n = 15 trials from 3 male mice. These experiments were performed with GCaMP6f labeling. All bar graphs and error bars represent mean ± s.e.m.
