Supplementary Figure 10: Local injection of muscimol into M1 impairs urination; and histological confirmation of ablation of M1 neurons in the area corresponding to the region identified by PRV. | Nature Neuroscience

Supplementary Figure 10: Local injection of muscimol into M1 impairs urination; and histological confirmation of ablation of M1 neurons in the area corresponding to the region identified by PRV.

From: A corticopontine circuit for initiation of urination

Supplementary Figure 10

a, Schematics of the experimental protocol. b, Left, schematics showing the injection sites. Right, post-hoc histology showing the injection site. c, Quantification of the effects of muscimol or ACSF injection into different brain regions on urination(muscimol into M1: 12 male mice; ACSF into M1: 10 male mice; muscimol into Ma: 10 male mice; muscimol into the PMC: 10 male mice; ACSF into the PMC: 10 male mice; one-sided Wilcoxon signed-rank test for muscimol group compared with washout group or one-sided Wilcoxon rank-sum test for the other comparisons (***P = 0.000034, 0.000031, 0.000073, **P = 0.0012, 0.0028; z = −3.039, −3.986, −4.004, −2.772, −3.796; df = 11, 20, 20, 9, 18 from left to right). d, Quantification of the effects of muscimol or ACSF injection into the M1 of female mice on urination (muscimol into M1: 7 female mice; ACSF into M1: 7 female mice). One-sided Wilcoxon rank-sum test, ***P = 0.0008 (z = −3.154; df = 12). e, Serial coronal sections showing the NeuN staining in red and DAPI in blue in the M1 area 4 weeks after co-injection of AAV-CaMKII-Cre-GFP and AAV-DIO-taCasp3. This experiment was repeated independently in 11 male mice with similar results. All bar graphs and error bars represent mean ± s.e.m.

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