Supplementary Figure 6: Partial loss of miR-137 results in impaired social behaviors. | Nature Neuroscience

Supplementary Figure 6: Partial loss of miR-137 results in impaired social behaviors.

From: Partial loss of psychiatric risk gene Mir137 in mice causes repetitive behavior and impairs sociability and learning via increased Pde10a

Supplementary Figure 6

a, Representative data for social interactions in Mir137loxP/+ (n = 10 mice) and Mir137loxP/+;Nestin-Cre (n = 8 mice) mice. Mir137loxP/+;Nestin-Cre mice exhibited relatively lower levels of following (t = 1.104, P = 0.2859), crawling over/under (t = 1.522, P = 0.1474) and sniffing (Nose-to-nose: t = 2.266, P = 0.0377; Anogenital t = 1.888, P = 0.0773), and higher levels of self-grooming (t = 3.164, P = 0.0060) than Mir137loxP/+ mice, Data shown are means ± s.e.m. Unpaired Two-Tailed t-test; n.s., nonsignificant; *, P < 0.05; **, P < 0.01. b, In 3-chamber test, both Mir137loxP/+ (n = 14 mice; t = 0.1743, P > 0.9999) and Mir137loxP/+;Nestin-Cre (n = 15 mice; t = 0.2245, P > 0.9999) mice had no preference for either left chamber or right chamber during the habituation phase. Data represent means ± s.e.m; Two-way ANOVA with Bonferroni post hoc test; n.s., nonsignificant. c–e, In the 3-chamber test, Mir137+/– mice exhibited impaired social behavior. In miR-137 gKO mice, both Mir137+/+ (n =9 mice) and Mir137+/– (n = 12 mice) mice had no preference for either left chamber (t = 1.177, P > 0.9999) or right chamber (t = 0.598, P > 0.9999) during the habituation phase (c), but Mir137+/– mice lacked a social preference for a mouse over an object (Mir137+/+ mice: tEmpty vs. Stranger1 = 2.857, P = 0.0414; Mir137+/– mice: tEmpty vs. Stranger1 = 0.8122, P > 0.9999; Mir137+/+ vs. Mir137+/–: tStranger1 = 3.052, P = 0.0248) (d). Moreover, unlike Mir137+/+ control mice, Mir137+/– mice displayed impaired social novelty recognition by demonstrating no preference for a novel mouse over a familiar mouse (Mir137+/+ mice: tStranger2 vs. Stranger1 = 3.496, P = 0.0351; Mir137+/– mice: tStranger2 vs. Stranger1 = 0.9823, P > 0.9999; Mir137+/+ vs. Mir137+/–: tStranger2 = 3.496, P = 0.0073) (e). Data represent means ± s.e.m; Two-way ANOVA with Bonferroni post hoc test; n.s., nonsignificant; *, P < 0.05; **, P < 0.01. f–g, In the social discrimination test, the Mir137+/– mice (n = 15 mice) exhibited impaired social discrimination ability. When compared with control mice, heterozygous cKO and gKO mice exhibited significantly fewer entries number (Mir137+/+ mice: tNovel vs. Familiar = 1.157, P > 0.9999; Mir137+/– mice: tNovel vs. Familiar = 0.2959, P > 0.9999; Mir137+/+ vs. Mir137+/–: tNovel = 2.744, P = 0.0489) (f) and lower % time spent (Mir137+/+ mice: tNovel vs. Familiar = 2.742, P = 0.0490; Mir137+/– mice: tNovel vs. Familiar = 1.598, P = 0.6943; Mir137+/+ vs. Mir137+/–: tNovel = 3.64, P = 0.0036) (g) in novel mouse zone. Data represent means ± s.e.m; Two-way ANOVA with Bonferroni post hoc test; n.s., nonsignificant; *, P < 0.05; **, P < 0.01.

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