Supplementary Figure 13: miRNA-2115 engineered to target ORC4 influences radial glia development.
From: Regulation of cell-type-specific transcriptomes by microRNA networks during human brain development
(a) Schematic shows mouse in vivo experimental design. Mouse cortex two days after electroporation at E13.5. White arrowhead indicates example of a dividing GFP+ radial glia cell, while yellow arrowheads indicate dividing intermediate progenitor cells. **-p<0.01, unpaired student t-test. (b) Related to Figure 4d—examines the impact of microRNA perturbation on radial glia number. We compared the proportions of SOW2 positive cells in control GFP expressing cells versus specific microRNA overexpression (miR1 or miR-2115) or specific anti-miR conditions. This analysis showed strong impact of miR-2115 on radial glia development. Interestingly, inhibition of miR-9 also lead to an increased proportion of SOX2 positive cells, consistent with previous studies68. This data was generated for three biological replicates (individuals, GW15, 17, and 19). Statistical significance was confirmed using Tukey’s test, with post hoc student t-test. P - *<0.05.
